Journal: ACS biomaterials science & engineering

Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.

doi: 10.1021/acsbiomaterials.4c01594

Figure Lengend Snippet: Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of rat2 fibroblasts grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.

Article Snippet: For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

Techniques: Electron Microscopy, Staining